Bovine pancreatic deoxyribonuclease

J. Stuuiiujw, 8. Moure, anti "w. Stein Compsuon of multiple forms of bovine pancreatic deoxyribonuclease. J. BioL Chan. 245 5685-5690 (1970). 

T. Hugh. Hie preparation and characterization of an active derivative of bovine pancreatic deoxyribonuclease A formed by selective cleavage with a-chymotrypsin. J. BioL Chem. 24 1712-1718 (1973). 

P. A. Price, S. Moore, and W- H. Stein. Alkylation of a histidine residue at the active site of bovine pancreatic deoxyribonuclease. J. Biot. Chem. 244 924-928... 

E. Junowicz and J. H. Spencer. Studies on bovine pancreatic deoxyribonuclease A 1. General properties and activation with different bivalent metals. Biochim. Biophys. Acta 312 72-84 (1973). 

T. L. Poulos and P. A. Price. Some effects of calcium ions on the structure of bovine pancreatic deoxyribonuclease A. J. BioL Chem. 247 2900-2904 (1972). 

T.-H. Liao, H.-C. Ho, and A. Abe. Chemical modification of bovine pancreatic deoxyribonuclease with phenylglyoxal—the involvement of Arg-9 and Arg-41 in substrate binding. Biochim. Biophys. Acta 1079 335-342 (1991). 

The role of the divalent metal ions present in natural phosphodiesterases became clear in bovine pancreatic deoxyribonuclease I (DNase I), the first endonuclease structure determined by X-ray crystallography. The nucleophilic attack of a water molecule activated by a histidine residue is facilitated by the interaction of a calcium ion with the phosphate group to be cleaved (291). Glutamic and aspartic residues involved in magnesium binding have been identified in the crystal structure of four type II restriction enzymes EcoRl (292), EcoRV (293), Pvull (294), and BamHl (295), as well as in that of the repair... 

Price PA (1975) The essential role of Ca in the activity of bovine pancreatic deoxyribonuclease. J Biol Chem 250 1981-1986... 

NADP+ oxidoreductase G6PDH from Leuconostoc mesenterioides Bovine pancreatic deoxyribonuclease F... 

Bovine pancreatic deoxyribonuclease (DNase I) is an endonuclease that cleaves both ssDNA and dsDNA to produce primarily 5 -P dinucleotides and 5 -P oligonucleotides (Fig. 3.1). DNase I degrades dsDNA in a sequence-nonspecific manner. The enzyme requires divalent cations (both Ca and Mg or Mn ) as cofactors for full double-strand cutting activity. In the presence of Mg alone as a cofactor, DNase I exhibits nicking activity and the single-strand nucleolytic activity is more discriminatory. 

Bovine pancreatic deoxyribonuclease 2 of the 3 Trp-residues reacted in the native enzyme, with 25% loss of activity 315)... 

Paulos, T. L., and P. A. Price Identification of a Tryptophan Residue Essential to the Catalytic Activity of Bovine Pancreatic Deoxyribonuclease. J. Biol. Chem. 246, 4041-4045 (1971). 

Bacterial nucleic acids were hydrolyzed using bovine pancreatic ribonuclease and deoxyribonuclease.37 Deoxyribonuclease treatment of disrupted bacterial suspensions has also been reported.38... 

Fig. 1. Chromatography of bovine pancreatic juice on DEAE-cellulose (anionic proteins) and Amberlite IRC-50 (cationic proteins) (1). RNAase, ribonuclease ChTg-a, chymotrypsinogen A Tg, trypsinogen ProCp-B and Cp-B, procarboxypeptidase B and carboxypeptidase B DNAase, deoxyribonuclease ProCp-A, procarboxypeptidase A.

Deoxyribonuclease I, DNase I (EC 3.1.21.1) an enzyme (normally obtained from bovine pancreas and therefore also known as pancreatic deoxyribonuclease) which catalyses random endonucleolytic cleavage of intemucleotide bonds of double-stranded DNA, preferentially but not exclusively between adjacent purines and pyrimidines, producing S -phos-phodi- and -oligonucleotides. 

Miscellaneous.—An e db-j8-D-acetamidodeoxyglucanase obtained from culture filtrates of Streptomyces griseus rapidly released oligosaccharides from Saccharo-myces cerevisiae )8-fructofuranosidase, bovine pancreatic ribonuclease I and deoxyribonuclease 1, and sulphitolysed ovalbumin these glycoproteins contain a D-mannopyranosyl residue attached to di-iV-acetylchitobiose, which, in turn, is linked to an L-asparagyl residue. Analysis of the hydrolysis products showed that the enzyme cleaved between the 2-acetamido-2-deoxy-D-glucopyranosyl residues. 

The oligosaccharide chains of bovine pancreatic ribonuclease I and deoxyribonuclease I have been cleaved, using a specific e/idb-jS-D-acetamidodeoxy-glucanase, although one of the two 2-acetamido-2-deoxy-D-glucopyranosyl residues was not detached from the protein chain. ... 

Anti-(human parotid a-amylase) and anti-(bovine-spleen deoxyribonuclease II) antibodies have been bound to various types of glass bead and challenged with human-parotid or pancreatic a-amylases or with deoxyribonuclease II, respectively. Although the beads in a column bound these enzymes, the amounts of the enzymes recovered from the beads were not quantitative and were extremely variable. U.v.-active material was also eluted. The bound anti-(a-amylase) antibody was used to form an active insoluble derivative of the homologous antigen. 

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