Delayed acid generation

Fig. 3. Phase Locked IR Pulses Time domain interferometry. (A) Output IR pulses from two tunable OPA-DFGs in the 4-pm frequency regime. (B) Three examples of interferograms generated by these IR pulses. (C) Linear IR absorption spectrum of acetic acid overlapped with the output of two OPAs. (D) Photon echo signal from acetic acid upon t-scan. The x-axis is the delay of the translation stage and the insert is a blow-up of a small region.

Acetylcholineesterase Bilayer lipid membranes were prepared by adding a solution of egg phosphatidylcholine and dipalmi-toyl phosphatidic acid dropwise into the surface of aqueous 0.1 M KC1/10 mM HEPES, near the Saran Wrap partition of a two compartment plexiglass cell. A portion of AChE solution in 10 mM Tris hydrochloride buffer solution of pH 7.4 was applied. The electrolyte level was momentarily dropped below the orifice and raised to form a membrane. The membranes were used as transducers for the reaction of AChE with ACh. An external voltage (25 mV) was applied across the membrane between two Ag/AgCl reference electrodes. Enzymatically generated hydronium ion causes transient current due to alteration of the electrostatic field by the ionization of dipalmitoyl phosphatidic acid. The response delay time was directly related to the substrate concentration where acetylcholine can be determined from 1 pM upto mM level. [113]... 

---