Dehydrogenases biochemical data

Biochemical data for his serum were as follows total bilirubin 99.2 pmol/L (normal is 3.4-20.5 pmol/L) conjugated bilirubin 15-4 pmol/L (normal is 0-6.8 pmol/L) alanine aminotransferase 125 U/L (normal is 0-40 U/L) and lactate dehydrogenase 570 U/L (normal is 120-250 U/L). 

Figure 1. Proposed metabolic branching points to various phenylpropanoid pathway derivatives based on existing chemotaxonomic / biochemical data. a. Phenylalanine ammonia-lyase, b. Tyrosine ammonia-lyase, c. Cinnamate-4-hydroxylase, d. Hydroxylase, e. 0-Methyltransferase, f. Cinnamoyl-CoA NADP oxidoreductase, g. Cinnamyl alcohol dehydrogenase.

Table 1 Biochemical Data of Some Commercially Available Dehydrogenases and Carbonyl Reductases...

Biochemical assays have been employed to measure changes in enzyme levels (e.g., aspartate aminotransferase, lactic dehydrogenase) as an indication of exposure to 1,2-dibromoethane in humans and animals (Albano et al. 1984 Botti et al. 1989 Letz et al. 1984 Van lersel et al. 1988). Decreased sperm counts per ejaculate and increased numbers of sperm with abnormal morphology have also been identified in workers following exposure to 1,2-dibromoethane (Ratcliffe et al. 1987 Wyrobek 1984). In general, these techniques are nonspecific for 1,2-dibromoethane exposure (see Chapter 2). There are no data to indicate whether a biomarker, if available, would be preferred over chemical analysis for monitoring exposure to 1,2-dibromoethane. 

Horner DS, Heil B, Happe T, Embley TM (2002) Iron hydrogenases - ancient enzymes in modern eukaryotes. Trends Biochem Sci 27 148-153 Horner DS, Hirt RP, Kilvington S, Lloyd D, Embley TM (1996) Molecular data suggest an early acquisition of the mitochondrion endosymbiont. Proc R Soc Lond B Biol Sci 263 1053-1059 Urdfl, Hirt RP, Dolezal P, Bardonova L, Foster PG, Tachezy J, Embley TM (2004) Trichomonas hydrogenosomes contain the NADH dehydrogenase module of mitochondrial complex... 

FIGURE 3.27 Binding of NADH with rabbit muscle lactate dehydrogenase using various methods of presenting experimental data. [Graphs reconstructed from data by Ward and Winzor, Biochem. J., 215, 685 (1983).]... 

Frosolono and Pawlowski (1977) studied biochemical changes in various lung fractions prepared from rats exposed to phosgene at concentrations near to or above the LCtso. A number of enzymes showed decreased activity in all fractions these included p-nitrophenyl phosphatase, cytochrome c oxidase, ATPase and lactate dehydrogenase (LDH). The serum LDH rose. It was suggested that either inhibition of enzyme activity or loss of enzyme from cells would account for these changes. The data available did not allow... 

These data are in good agreement with data on the activity of 6-phosphoglu-conate dehydrogenase from the same individuals (Fig. 15). Therefore, in the case of dose compensation, the correlation between transcriptional activity and the biochemical expression of the trait was strictly demonstrated (Faizullin and Gvozdev, 1973). 

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