Deconvolution tailored

In this section, more general excitation patterns will be discussed, which allow for tailored deconvolutable excitations with high spectral power densities. Periodic amplitude modulation with a single frequency has been proposed in the literature [73]. It is well suited for noise suppression by lock-in detection, but in practice it suffers from stability problems during the slow frequency sweep. 

This approach was reported by Carell et al. [30] and, whilst it has often been cited with the name subtractive deconvolution, it could be also named tailored deconvolution. One of the libraries deconvoluted by this method, and targeted to trypsin inhibition, was prepared in solution adding equimolar amounts of 19 amino acids (Gin excluded) to a tetrafunctional xanthene scaffold, to produce a solution library composed of 65,341 individuals as a single pool (Figure 8.7). The extensive chemical and analytical assessment which assured the quality of the library included synthesis of six smaller model libraries (30-60 compounds), using simplified xanthene scaffolds, and their thorough MS characterization. Further details are not presented here, being out of the purpose of this review, but the conclusions about the library quality can be safely accepted. 

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