Deciphering a Complex Electroenzymatic Response Horseradish Peroxidase

Deciphering a Complex Electroenzymatic Response Horseradish Peroxidase 

Horseradish peroxidase (HRP) is an extracellular plant enzyme that acts in regulation of cell growth and differentiation, polymerization of cell wall components, and the oxidation of secondary metabolites essential for important pathogenic defense reactions. Because of these essential functions, and also because of its stability and ready availability, HRP has attracted considerable attention.13 It has been involved in a number of applications, such as diagnostic assays,14 biosensors,15 bioremediation,16 polymer synthesis,17 and other biotechnological processes.18 More applications in which HRP catalysis is translated into an electrochemical signal are likely to be developed in the near future. 

At relatively high concentrations of H202, its consumption may be regarded as negligible. Superposition of catalysis and inhibition then produces cyclic voltammetric responses that may be described by the following equation, in which 1ms represents a new function, as detailed in Section 6.5.2. 

The Ping-Pong Mechanism with an Immobilized Enzyme and the Cosubstrate in Solution 

Insofar as the reactions in the catalytic cycle are fast, we may assume that the various forms of the enzyme obey the steady-state approximation. 

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