DDT-ase

DDT Musca domestica Many strains Enhanced DDT-ase DDTase inhibitors reduce resistance... 

This feat of "accelerated microevolution" involved the genetically controlled production of a dehydrochlorinating enzyme ("DDT-ase") that converted DDT to DDE. Microsomal oxidation of the latter produced polar degradable compounds. 

Tao, S. et al.. Use of sequential ASE extraction to evaluate the bioavailabdity of DDT and its metabolites to wheat roots in soils with various organic carbon contents, Sci. Total Environ., 320, 1, 2004. 

Fig. 6.7. Extraction of DDT and its metabolites from aged contaminated soil. Empty bars correspond to ASE (three static cycles) and shaded ones to Soxhiet extraction. (Reproduced with permission of Elsevier.)...

According to the theory of Matsumura and Patil (1969), based on their experimental findings, DDT acts by inhibiting the Na, K and Mg ATP -ases. 

Other authors reported that, using three sequential static phases, ASE (here defined as RLE) removed an equivalent quantity of DDT and its metabolites as Soxhlet extraction in less time and with less solvent and that recovery was almost quantitative when the sample was appropriately worked-up and manipulated. Hubert and coworkers investigated the dependence of the extraction efficiency of POPs, including OCRs, on the ASE operating variables solvent and... 

Any of the common laboratory solvents can be used in the extractor, but the final choice will depend on the analytes that are to be extracted. Fritzpatrick and Dean described a method for selecting the optimum solvent for the extraction of DDT (and metabolites) and pentachlorophenol (PCP) from soil when using ASE they broke the Hildebrand solubility parameter down into three components, to optimally select methylene chloride for DDT and a mixmre of methylene chloride and acetonitrile for PCP (61). 

Matsumura and Marahashi found DDT to strongly inhibit Na, K, and Mg++ ATP ase of rat brain and have suggested a correlation between ATP -ase Inhibition and the electrophysiologleal symptoms of DDT poisoning. 

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