Data evaluation densitometry

Quantitative analyses are obtained by densitometry. The TLC scaimer used for this is linked to a personal computer (PC) and is controlled by an evaluation program. The PC performs the calculation of the result, supports the protocol, and provides an archive of all the parameters of the equipment and evaluation program, the raw data and the numerical and graphical results. 

The cellulose layers have several advantages e.g., they are stable, they can be used with various specific reagents, and they give reproducible data. They are recommended particularly for quantitative evaluation by densitometry. The drawbacks of cellulose layers are that corrosive reagents cannot be used and the sensitivities of detection reactions of certain amino acids are lower than on silica gel layers. 

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