Dansyl-glutathione

Gan, J. et al. 2005. Dansyl glutathione as a trapping agent for the quantitative estimation and identification of reactive metabolites. Chem. Res. Toxicol. 18 896. 

FIGURE 14.1 The stractures of glutathione and its conjugate, Af-acetylcysteine and its conj ugate, (25,3ff)-3-(4-hydroxyphenyl)-2-[4-(2-piperidin-1 -ylethoxy)phenyl]-2,3-dihy-dro-l,4-benzoxathiin-6-ol (A) and its bis-cyano adduct (B), (25,3/f)-(-I-)-3-(3-hydro-xyphenyl)-2-[4-(2-pyrrolidin-l-ylethoxy)-phenyl]-2,3-dihydro-l,4-benzoxathiin-6-ol (C) and its JV-acetylcysteine adduct (D), dansyl glutathione, /f-(-t )-pulegone, menadione, and iodoacetamide. 

Protocol for Qualitative and Quantitative Analysis of Thiol Adducts Using Dansyl Glutathione (dGSH)... 

Preparation of dGSH Dansyl glutathione (Fig. 14.1) is prepared by derivatization of glutathione disulfide (GSSG) with dansyl chloride and subsequent cleavage of the disulfide bond by dithiothreitol (Gan et al., 2005). 

Jones, D.P., Carlson, J.L., Samiec, P.S., Sternberg, P. Jr., Mody, V.C. Jr., Reed, R.L. et al. (1998) Glutathione measurement in human plasma evaluation of sample collection, storage and derivati-zation conditions for analysis of dansyl derivatives by HPLC. Clin. Chim. Acta, 275, 175-184. 

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