Dansic acid

Draw a picture in your notebook of the polyamide thin-layer plate exposed under UV light after each of the two or three solvent developments. These pictures should look similar to Figure E2.7. Three fluorescent areas should be evident after solvent 2 however, better separation is achieved by solvent 3. A blue fluorescent area at the bottom of the plate is dansic acid (DNS-OH), which is a hydrolysis product of dansyl chloride. A blue-green fluorescent spot about one-third to one-half up the plate is dansyl amide (DNS-NH2), which is produced by reaction of dansyl chloride with ammonia. A third spot, which usually fluoresces green, is the dansyl derivative of the NH2-terminus amino acid. Note the positions of the standard dansyl amino acids and compare with the unknown. What is the identity of the NH2-terminal amino acid Are any other fluorescent spots evident on the plate Using polarity or nonpolarity, try to explain the position of each molecule on the thin-layer plate. 

B 7 In the TLC analysis of dansyl amino acids, why does dansic acid move very slowly during development in solvents 2 and 3 ... 

Dansic acid is very polar in fact, it may be ionized during TLC analysis. 

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See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

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