Cytochrome P450 reductase plant

Cinnamate 4-hydroxylase (C4H EC 1.14.13.11, also defined as CYP73A [36]) catalyzes the p-hydroxylation of trani-cinnamate to form trani -p-coumarate. The enzyme has a requirement for molecular oxygen and NADPH as well as association with the electron donor NADPH-cytochrome P450 reductase (CPR EC 1.6.2.4) for activity. C4H was the first characterized plant P450 [37, 38]. 

Yeast strain for the co-expression of a plant cytochrome P450 monooxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase and use thereof in bioconversion... 

Mao, W., Rupasinghe, S.G., Zangerl, A.R., Berenbaum, M.R. and Schuler, M.A. (2007) Allelic variation in the Depressaria pastinacella CYP6AB3 protein enhances metabolism of plant allelochemicals by altering a proximal surface residue and potential interactions with cytochrome P450 reductase. /. Biol. Chem., 282,10544—52. 

Ro, D. Ehlting, J. Douglas, C.J. Cloning, functional expression, and subcellular localization of multiple NADph-cytochrome P450 reductases from hybrid poplar. Plant Physiol. 2002, 130, 1837-1851. 

Benveniste, I., A. Lesot, M.P. Hasenfratz, G. Kochs, and F. Durst (1991). Multiple forms of NADPH-cytochrome P450 reductase in higher plants. Biochem. Biophys. Res. Commun. 177, 105-112. 

First, Ro and Douglas constructed the initial steps of the plant phenylpropanoid pathway by connecting two enzymes, PAL and C4H, with a cytochrome P450 reductase (CPR) from a plant source in S. cerevisiae [31]. This heterologous transfer of plant phenylpropanoid pathway genes into yeast became the platform for the biosynthesis of various flavonoids and related compounds in microbial... 

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