Cytochrome P450 reductase antibodies

In the catalytic cycle of CYP, reducing equivalents are transferred from NADPH to CYP by a flavoprotein enzyme known as NADPH-cytochrome P450 reductase. The evidence that this enzyme is involved in CYP monooxygenations was originally derived from the observation that cytochrome c, which can function as an artificial electron acceptor for the enzyme, is an inhibitor of such oxidations. This reductase is an essential component in CYP-catalyzed enzyme systems reconstituted from purified components. Moreover antibodies prepared from purified reductase are inhibitors of microsomal... 

Shen, S. and H.W. Strobel (1994). Probing the putative cytochrome P450- and cytochrome c-binding sites on NADPH-cytochrome P450 reductase by anti-peptide antibodies. Biochemistry 33, 8807-8812. 

In the reconstituted systems the reductase proved not to be specific for a particular cytochrome P450. The enzyme from liver could reduce the to-monooxygenase from kidney and vice versa and its antibody was effective in inhibiting the NADPH-mediated cytochrome c reduction in spleen microsomes. 

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