Cytochrome P450 monooxygenase yeast

Yeast strain for the co-expression of a plant cytochrome P450 monooxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase and use thereof in bioconversion... 

A feature of some pterocarpan phytoalexins (e.g., pisatin and glyceollin of pea and soybean, respectively) is their hydroxylation at position 6a, a reaction catalyzed by a microsomal cytochrome P450 monooxygenase.49 50 A cDNA encoding this enzyme was recently characterized from elicited soybean cell cultures.51 The microsomal protein, expressed in yeast cells, catalyzed the stereoselective hydroxylation of (6a/ , lla/ )-3,9-dihydroxypterocarpan to its 6a-hydroxy derivative. It was also demonstrated that the enzyme expression is regulated at the transcriptional level.51... 

T.J. Fleischmann, and R.E. Dewey (1999). Expression of a soybean cytochrome P450 monooxygenase cDNA in yeast and tobacco enhances the metabolism of phenylurea herbicides. Proc. Natl Acad. Sci USA 96, 1750-1755. 

Van Bogaert IN, De Mey M, Develter D, Soetaert W, Vandamme EJ (2009) Importance of the cytochrome P450 monooxygenase CYP52 family for the sophorolipid-producing yeast Candida bombic-ola. FEMS Yeast Res 9 87-94... 

A notable exception to the voltammetric transduction of P450 has been reported by Kara et al. [312]. The authors used a fusion enzyme between rat P450 lAl monooxygenase and yeast NADPH-cytochrome P450 oxidoreduc-tase expressed in Saccharomyces cerevisiae that is immobilized in an agarose layer on the gate of an ISFET for the monitoring of the o-deethylation of 7-ethoxycoumarin to 7-hydroxycoiunarin. As depicted in Eq. (10.12), the... 

Fe(IV) is an important intermediate in such heme enzymes as cytochrome c oxidase and cytochrome P450, as well as the nonheme enzymes methane monooxygenase and ribonucleotide reductase. Voltammetric studies on yeast cytochrome... 

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