Cytochrome engineering

Many enzymes have been the subject of protein engineering studies, including several that are important in medicine and industry, eg, lysozyme, trypsin, and cytochrome P450. SubtiHsin, a bacterial serine protease used in detergents, foods, and the manufacture of leather goods, has been particularly well studied (68). This emphasis is in part owing to the wealth of stmctural and mechanistic information that is available for this enzyme. 

Mauk AG (1991) Electron Transfer in Genetically Engineered Proteins. The Cytochrome c Paradigm.75 131-158... 

Wolfbeis OS, Reisfeld R, Oehme 1 (1995) Sol-Gels and Chemical Sensors. 85 51-98 Wong L-L, Westlake ACG, Nickerson DP (1997) Protein Engineering of Cytochrome P450,am. 88 175-208... 

Cosme J, Johnson EF. Engineering microsomal cytochrome P450 2C5 to be a soluble, monomeric enzyme. Mutations that alter aggregation, phospholipid dependence of catalysis, and membrane binding. /FtoZ Chem 2000 275 2545-53. 

Jones JP, EJ O Hare, L-L Wong (2001) Oxidation of polychlorinated benzenes by genetically engineered CYPlOl (cytochrome P450, jjj). Eur J Biochem 268 1460-1467. 

Fasan, R., Chen, M.M., Crook, N.C. et al. (2007) Engineered alkane-hydroxylating cytochrome P450 (BM3) exhibiting nativelike catalytic properties. Angewandte Chemie (International Edition in English), 46 (44), 8414-8418. 

Rushmore, T.H., Reider, P.J., Slaughter, D. et al. (2000) Bioreactor systems in drug metabolism synthesis of cytochrome P450-generated metabolites. Metabolic Engineering, 2, 115-125. 

Landwehr, M., Hochrein, L., Otey, C.R. et al. (2006) Enantioselective a-hydroxylation of 2-arylacetic acid derivatives and buspirone catalyzed by engineered cytochrome P450 BM-3. Journal of the American Chemical Society, 128, 6058-6059. 

Leonard, E. and Koffas, M.A. (2007) Engineering of artificial plant cytochrome P450 enzymes for synthesis of isoflavones by Escherichia coli. Applied and Environmental Microbiology, 73, 7246—7251. 

WERCK-REICHHART, D., HEHN, A., DIDIERJEAN, L Cytochromes P450 for engineering herbicide tolerance, Trends Plant. Sci., 2000,3, 116-123. 

Kumar, S., Scott, E. E., Liu, Hong, and Halpert, J. (2003) A rational approach to re-engineer cytochrome P450 2B1 regioselectivity based on the crystal structure of cytochrome P450 2C5. 278, 17,178-17,184. 

Kamataki, T. et al. (2002) Role of human cytochrome P450 (CYP) in the metabolic activation of nitrosamine derivatives application of genetically engineered Salmonella expressing human CYP. Drug Metab. Rev, 34 (3), 667-676. 

Murakami, H., Yabusaki, Y., Sakaki, T., Shibata, M. Ohkawa, H. (1987). A genetically engineered P450 monooxygenase construction of the functional fused enzyme between rat cytochrome P450c and NADPH-cytochrome P450 reductase. DNA, 6, 189—97-... 

Sakaki,T., Kominami, S., Takemori, S., Ohkawa, H., Akiyoshi-Shibata, M. Yabusaki, Y. (1994). Kinetic studies on a genetically engineered fused enzyme between rat cytochrome P4501A1 and yeast NADPH-P450 reductase. Biochemistry, 33, 4933-9. 

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