Cytidine 5 - , sodium salt

C19H22N7Na013P 8H20 Guanylyl-(3 — 5 )-cytidine, sodium salt,... 

C14H25N4OuP2 Na+ 4 H20 Cytidine 5 -(choline diphosphate), sodium salt, tetrahydrate (CYPCHOOl)304... 

Methylcytidine, 3-deazacytidine/ l-P-o-arabinofuranosylcytosine 2, 5 -phosphate/ cytidine 5 -diphosphocholine(sodium salt), thymidine 3, 5 -(dimethylphosphoramidate), 4-a-D-erythrofuranosyl-1 (4-tolyl)imidazo-... 

An interesting new procedure was employed in the synthesis of L-adenosine, L-guanosine, L-cytidine, L-uridine, L-5-methyluridine, and L-6-azauridine, involving the reaction of 2-0-tosyl-5-0-trityl-L-arabinose (I) with a suspension of the sodium salt of the appropriate base in DMF, to give 32-92% yields of the 5 trityl derivatives of the corresponding L-p-ribofuranosides (111). The reaction presumably proceeds through an epoxide intermediate (II) resulting from the action of the base on 1. ... 

Prepare individual standards (Sigma) of adenosine-5 -phosphoric acid, cytidine 5 -phosphate (sodium salt), guanosine-5 -phosphate (sodium salt), and uridine-5 -phosphate (disodium salt) as l-pg/p. solutions in deionized water. Prepare each standard by dissolving 10 mg of the nucleotide in 10 ml of water. Prepare a mixed standard of the four nucleotides at 1 Ag/)il for each. To obtain the mixed standard, weigh 10 mg of each nucleotide and dissolve in 10 ml of water to achieve a concentration of 1 )ig/ J.l for each. 

The following separations (approximate values included) will be achieved for both individual nucleotides and the mixed standard guanosine-5 -phosphate (sodium salt), 0.34 adenosine-5 -phosphoric acid, 0.48 cytidine-5 -phosphate (sodium salt), 0.60 and uridine-5-phosphate (disodium salt), 0.74. The compounds will appear as dark spots against a bright fluorescent background at 254 nm. 

The 5,6-double bond in uracil, 5-fluorouracil, /V-alkyluracils, thiouracils, and uridines adds sodium sulfite or bisulfite to give the corresponding 5,6-dihydro-6-sulfonic acid salts. Bisulfite addition to cytosines and cytidine may be succeeded by a second reaction involving nucleophilic replacement of the amino group, for example, by water. 

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