Cysteamine SAM

Hexacyanoferrates were immobilized on Au covered with SAM of 3,3 -thiodipropionic acid [86]. It has been found from voltammetric studies that the surface coverage of hexacyanoferrate is close to one monolayer and such an electrode exhibits very good surface redox behavior. Cheng et al. [87] have described the formation of an extremely thin multilayer film of polybasic lanthanide heteropolytungstate-molybdate complex and cationic polymer of quaternary poly(4-vinylpyridine), partially complexed with osmium bis(2,2 -bipyridine) on a gold electrode precoated with a cysteamine SAM. Consequently, adsorption of inorganic species might also be related to the properties of SAMs. This problem will be discussed in detail in a separate section later. 

Fig. 3. Immobilization of antibody on the PZ resonator. Gold modified with cysteamine SAM was activated with glutaraldehyde, protein A was covalently attached and captured Ab.

Just briefly mentioned should be quinone dyes 28 [201], cyanine SAMs 29 where the cyanine dye forms highly ordered 2D J-aggregates on a cysteamine SAM with a 7 X V3 superlattice [ 145,146] and a perylene bisimide dye 30, which shows J-type aggregation as demonstrated by the bathochromic shift of the fluorescence spectra [202] (Fig. 33). 

Su et al. incorporated SAMs in the development of a Pz sensor for IgE [133]. Thioctic acid and cysteamine, coupled with EDC and glu-taraldehyde respectively, were tested. IgE was measured hnearly from 5 to 300 lU mL and the antibody-coated crystal could be regenerated five times without detectable loss in activity. The SAM coated crystals showed three... 

Fig. 14 Reaction steps involved in the surface modification process, (a) Coupling of biotin to the SAM of cysteamine through EDC catalyzed amidation. (b) Binding of streptavidin to the preformed biotin layer, (c) Binding of the glutamate transporter to the streptavidin/biotin layer via the genetically engineered strep-tag at the N-terminus of the membrane protein. [Reprinted from ref 100 by permission of the Royal Society of Chemistry copyright 2008.]...

Fig. 11 The time-dependent profile (mean and standard deviation) of the normalized impedance response of the self-assembled monolayers (SAMs) of m-amine alkanethiols, cysteamine (CA)-laminin-modified IME chip (a) cultured with PC-12 cells ( = 6) and (b) PC-12 cells exposed to S mM nifedipine...

Figure 14.4 (A) EIS complex plane plots obtained in the presence of 5 mM PBQ/H2Q redox probe on the gold cysteamine (Au-CA)-nitrilodihydroxamic acid (NDHA) self-assembled monolayer (SAM) electrode in 0.1 M acetate buffer solution (ABS), pH3.5, and 0.1 M NaCIO,f, after 20 min of accumulation of Zr(IV) from 0.23 M HCIO solutions containing (a) O.OM, (b) I.Ox 10- M, (c)5.0x KT M, (d) l.Ox W M, (e)5.0x lO M, (f) I.Ox 10 M, (g) 5.0X 10- M, (h) I.Ox 10- M, (i) 5.0x 10 M, (j) I.Ox lO M, and (k) 5.0x 10 M Zr(IV). (B) The inset shows the calibration curve obtained from complex plane plots fAR, = - Rctftj- From Ref [17] with permission from Elsevier...

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