1- Cyclohexyl-3- -carbodi

Calf thymus (CT) DNA was first fragmented by sonication, then purified by a standard procedure. Agarose gel electrophoresis for the sonicated CT DNA revealed a distribution in molecular weight ranging from 30 kDa to 260 kDa which is equivalent in base pairs (bp) from 40 to 400 (1 bp = 660 Da). The DNA fragments were reacted with HEDS in the presence of l-cyclohexyl-3-(2-morpholinoethyl) carbodi-imide metho-p-toluenesulfonate (CMC/jTs). Finally, the reaction mixture was gel-filtered and the macro-molecular fractions, when displayed the characteristic absorption of 260 nm of the nucleic bases, were collected. 

The racemic tetracyclic amine (3) can be coupled to ( )-(+)-p-tolylsulfinylacetic acid using the modified carbodi-imide reagent l-Cyclohexyl-3-(2-morpholinoethyl)carbodiimide Metho-p-toluenesulfonate to give the diastereomeric sulfinyl amides (4) and (5) (eq 2), which are readily separated by HPLC. ... 

Cyclohexyl-3-(2-morpholinoethyl)-carbodi-imide, metho-p-toluensulfonatc, 227... 

When the binding reaction is carried out in aqueous medium, water-soluble carbodi-imides should be used, e.g. N-cyclohexyl-N-[2-(N-methylmorpholino)ethyl]carbo-diimide p-toluenesulfate. 

Ultrogel , a commercially available polyacrylamide-agarose medium, has been used as support matrix for the coupling of D-tryptophan methyl ester in a comparative study of the efficacy of two condensation agents, l-cyclohexyl-3-(2-morpholinoethyl)-carbodi-imide-metho-4-toluene sulphonate and A-ethoxy-... 

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