Cyclodextrin glucanotransferase

Cyclobuxine-D, 2 104 P-Cyclocitral, 24 570 Cyclocitronellene acetate, 24 488 a-Cyclocitrylidenebutanone, 24 565 Cyclodehydrating agents, 20 276 Cyclodemol, 24 488 P-Cyclodextrin, 4 715 Cyclodextrin glucanotransferase, 24 48 Cyclodextrin glycosyltransferase, 4 715 Cyclodextrin inclusions, 74 183 Cyclodextrin inclusion compounds, 74 166-167... 

As the second century of cyclodextrin research begins, attention is being focused on the biochemistry of CDs and their biocatalysts, cyclodextrin glucanotransferases (CGTases). Recent advances in protein structure and... 

The distribution of a-, P- and y-CDs is highly dependent on the origin of biocatalyst used (See section on Cyclodextrin Glucanotransferases). Product distributions maybe altered by the addition of specific precipitants, such as aromatics and long chain alcohols 9, 20), Depending on molecular size, these precipitants preferentially complex with specific CD species and are removed from solution. 

Cyclodextrin glucanotransferase (CGTase) [EC 2.4.1.19] is one of the more unusual members of the amylolytic glucosylase family. Whereas most... 

Vassileva, A., Burhan, N., Beschkov, V. et al.. Cyclodextrin glucanotransferase production by free and agar gel immobilized cells of Bacillus circulans ATCC 21783, Process Biochem., 38 (2003) 1585-1591. 

R. Kanai, K. Haga, K. Yamane, and K. Harata, Crystal structure of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011 complexed with 1-deoxynojirimycin at 2.0 A resolution,. /. Biochem., 129 (2001) 593-598. 

In 2002, Do et al. [85] proposed a pathway for the enzymatic synthesis of (-)-menthyl a-maltoside and a-maltooligosides from (-)-menthyl a-glucoside using cyclodextrin glucanotransferase obtained from Bacillus macerans. The reaction can be performed in a reactor containing (-)-menthyl a-glucoside, the enzyme and soluble starch the yield was about 80% 15% (-)-menthyl a-malto-side and 65% (-)-menthyl a-maltooligosides, respectively. Treatment of the starch with a-amylase can raise the proportion of (-)-menthyl a-maltoside. 

Amino acid sequence relationships between that of branching enzyme (BE) and amy-lolytic enzymes, such as a-amylase, pullulanase, glucosyltransferase and cyclodextrin glucanotransferase, especially at those amino acids believed to be contacts between the substrate and the amylase family enzymes, were first reported by Romeo et al.283 Baba et al.284 reported that there was a marked conservation in the amino acid sequence of the four catalytic regions of amylolytic enzymes in maize endosperm BEI. As shown in Table 4.12, four regions that putatively constitute the catalytic... 

Terada, Y., Yanase, M., Takata, H., Takaha, T., and Okada, S. 1997. Cyclodextrins are not the major cyclic a-1,4-glucans produced by the initial action of cyclodextrin glucanotransferase on amylase./. Biol. Chem.,25,15729-15733. 

Cyclodextrins (see Chap. 5) can be prepared in one step from starch using a recombinant enzyme based on a thermophilic bacterium, a species of Thermoanaerobacter,254 Cyclodextrin glucanotransferase from Bacillus macerans and other organisms can also be used.255... 

Nakano, H., and Kitahata, S. 2005. Application of cyclodextrin glucanotransferases to the synthesis of oligosaccharides and glycosides. In Handbook of Industrial Biocatalysis, ed. C.T. Hon. Boca Raton, FL CRC Press, Taylor Francis Group, pp. 22-1-15. 

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