Glucanotransferases

Cyclobuxine-D, 2 104 P-Cyclocitral, 24 570 Cyclocitronellene acetate, 24 488 a-Cyclocitrylidenebutanone, 24 565 Cyclodehydrating agents, 20 276 Cyclodemol, 24 488 P-Cyclodextrin, 4 715 Cyclodextrin glucanotransferase, 24 48 Cyclodextrin glycosyltransferase, 4 715 Cyclodextrin inclusions, 74 183 Cyclodextrin inclusion compounds, 74 166-167... 

As the second century of cyclodextrin research begins, attention is being focused on the biochemistry of CDs and their biocatalysts, cyclodextrin glucanotransferases (CGTases). Recent advances in protein structure and... 

The distribution of a-, P- and y-CDs is highly dependent on the origin of biocatalyst used (See section on Cyclodextrin Glucanotransferases). Product distributions maybe altered by the addition of specific precipitants, such as aromatics and long chain alcohols 9, 20), Depending on molecular size, these precipitants preferentially complex with specific CD species and are removed from solution. 

Cyclodextrin glucanotransferase (CGTase) [EC 2.4.1.19] is one of the more unusual members of the amylolytic glucosylase family. Whereas most... 

Vassileva, A., Burhan, N., Beschkov, V. et al.. Cyclodextrin glucanotransferase production by free and agar gel immobilized cells of Bacillus circulans ATCC 21783, Process Biochem., 38 (2003) 1585-1591. 

R. Kanai, K. Haga, K. Yamane, and K. Harata, Crystal structure of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011 complexed with 1-deoxynojirimycin at 2.0 A resolution,. /. Biochem., 129 (2001) 593-598. 

An export-affinity fusion vector containing the gene cgt encoding cyclomaltodextrin glucanotransferase (CGTase) from Bacillus circulans var. alkalophilus has been described... 

In 2002, Do et al. [85] proposed a pathway for the enzymatic synthesis of (-)-menthyl a-maltoside and a-maltooligosides from (-)-menthyl a-glucoside using cyclodextrin glucanotransferase obtained from Bacillus macerans. The reaction can be performed in a reactor containing (-)-menthyl a-glucoside, the enzyme and soluble starch the yield was about 80% 15% (-)-menthyl a-malto-side and 65% (-)-menthyl a-maltooligosides, respectively. Treatment of the starch with a-amylase can raise the proportion of (-)-menthyl a-maltoside. 

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