Cyclic activity, bound lipases

Figure 5. Cyclic activity (pH 8.5, 3(fC) of bound lipase on cellulosefibers.

Long chain fatty acids derived from the adipose tissue are released through the action of the adipose tissue lipase (Vaughan et al., 1964) and the free fatty acids released from the adipose tissue are transported in the circulation bound to albumin. As it well known, lipase activity increases in response to p-adrenergic stimulation via the activation of adenyl cyclase and the second messenger, cyclic AMP (Butcher, 1972). On the other hand,... 

Enzymatic ROP of other cyclic monomers, l,4-dioxan-2-one, [56-58], 3(S)-isopropylmorpholine-2,5-dione and its derivatives have been studied [59, 60]. Out of 12 enzymes (7 lipases, 2 esterases and 3 proteases) studied, immobilised lipase from C. antarctica gave the best results. In a recent study [58], the activity of CALB was improved by coating with an ionic liquid, 1-butyl-3-methylimidazolium hexafluorophoshphate, which protected the bound water layer surrounding the lipase. Poly(l,4-dioxan-2-one) (PPDO) with a MW of 182,100 g/mol was obtained via this method. The thermodynamics and kinetics of CALB [57] showed that the polymerisation of l,4-dioxan-2-one reached equilibrium after 12 h at 60 C in the presence of 5wt% lipase, and the ROP kinetics of PPDO was of the first order with regards to the monomer concentration. 

The rdle of adipose tissue glyceride lipase in regulating plasma FFA levels, liver TG and plasma lipoprotein-bound lipids has prompted the investigation of drugs interfering with the activation of the lipolytic enzyme, by acting directly on it or by inhibiting the formation of cyclic 3, S -AMP in adipose tissue. 

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