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Cyanidin, HPLC analysis

In a polyphenolic extract, anthocyanins can interfere with other polyphenolics such as pro-cyanidins during HPLC analysis and hence should be removed prior to analysis. Anthocyanins from crude polyphenolic extracts can be removed as described in Basic Protocol 2. The ethyl acetate used for elution of phenolic compounds other than anthocyanins is removed using a rotary evaporator at 20°C. The non-anthocyanin polyphenolics are dissolved in deionized distilled water and the pH is adjusted to 7.0 with NaOH as described in Alternate Protocol 2 or the method developed by Oszmianski and Lee (1990a). In the latter method, polyphenolics were fractionated into three groups neutral fraction A (flavanols and other polar phenolics), neutral fraction B (flavonols), and acidic phenolics. Polyphenolic extracts were adjusted to pH 7.0 with NaOH... [Pg.1247]

See other pages where Cyanidin, HPLC analysis is mentioned: [Pg.162]    [Pg.259]    [Pg.854]    [Pg.53]    [Pg.515]    [Pg.2267]    [Pg.656]    [Pg.136]    [Pg.809]    [Pg.163]    [Pg.542]    [Pg.187]   
See also in sourсe #XX -- [ Pg.41 , Pg.42 , Pg.43 , Pg.44 ]



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Cyanidin

HPLC analysis

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