Cultivars compared

FIGURE 4.1 Seed yield of University of Manitoba HEAR cultivars compared to Reston, the first HEAR cultivar registered in Canada in 1982. 

Comparative Western analysis of samples directly extracted from tissue without intermediate purification [Pg.276]

Galvis-Sanchez AC, Gil-Izquierdo A and Gil MI. 2003. Comparative study of six pear cultivars in terms of their phenolic and vitamin C contents and antioxidant capacity. J Sci Food Agric 83 995-1003. 

HPLC/PDA/MS has also been used to compare the saponin profiles in multiple cultivars of alfalfa and M. truncatula. Comparative profiles are provided (Fig.3.8). It is interesting that these closely related legumes yielded different saponin profiles. The saponin profile of M. truncatula is more complex than alfalfa and may provide a richer source for mining putative pharmaceuticals. 

Figure 3.8 Comparative saponin profiles for two cultivars of alfalfa and one cultivar of M. truncatula obtained by reverse-phase HPLC/PDA/MS using electrospray ionization and an ion trap mass spectrometer. The profiles illustrate the increased complexity of saponins in M. truncatula and offer a richer source for bio-prospecting of natural products.

Walter et al. (38) measured the protein efficiency ratio (PER) of flour prepared from sweet potatoes which were cooked in a drying oven. Because the PER is determined on the basis of a diet containing 10% protein, the Jewel and Centennial sweet potatoes used in this study were stored until sufficient starch had metabolized to increase crude protein content to 11.25% (dry basis). When the flour was fed to Sprague-Dawley strain rats, the corrected PER values were 2.22 and 2.00 for Centennial and Jewel cultivars, respectively, compared to 2.50 for casein. Centennial had the highest PER value of the two cultivars because its NPN content was lower. The net effect of increased NPN content is to lower the amount of essential amino acids as a percentage of the total nitrogen and thus decrease the PER value. 

Figure 3 shows a gas chromatogram obtained from soil with plants of one barley cultivar, Etu. It is possible to count over 400 peaks, and the figure illustrates the very complex pattern of volatiles in the rhizosphere. If we compare chromatograms obtained from samples of Etu, Tellus and soil only, no specific difference can be seen. Most of the peaks seem to be present in all three chromatograms, although the intensities of the peaks vary. The necessity of computer help with the pattern recognition is obvious. 

In onion bulbs, if some growth of inner buds takes place already before irradiation, the treatment causes the death and discoloration of the inner buds. The area of this discoloration depends on the size of the inner buds at the time of irradiation. Pilot scale studies in Hungary have shown that the yield of unirradiated onions prepared for drying after storage was 29% of the original compared to 55% in irradiated onions. The dehydrated onions prepared from irradiated bulbs had better quality than did the controls. No discoloration of the inner buds occurred in bulbs of cultivar Alsogodi irradiated at 0.05 kGy and stored up to 8 months [25]. Similar results were reported earlier from Egypt [26] whereas some studies in the United States have indicated that inner-bud discoloration of irradiated onions may lower the quality of dehydrated onion slices or powder prepared from them [27]. 

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