Cubic lipid phase method

Gordeliy VI, Schlesinger R, Efremov R, Buldt G, Heberle J. Crystallization in lipidic cubic phases a case study with bacte-riorhodopsin. Methods Molec. Biol. 2003 228 305-316. 

PLB of the type found in maize are thought to be built up of lipid bilayers organised into a bicontinuous cubic phase based on a set of intersecting tubes following the geometry of the diamond lattice. It has been suggested that this type of structure constitutes a Schwartz minimal energy surface commonly referred to as a D-surface [1]. We have performed parallel electron microscopy and X-ray diffraction methods to test this hypothesis. 

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