Crystallization screening methodology

Sonication is also another method of generating co-crystals. It is applied to a solution or suspension. Although this technique is little used in co-crystal screening, a recent study has shown that pure co-crystals could be obtained using ultrasound assisted solution co-crystallization, where conventional solution crystallization failed. he use of melt crystallization methods have also been reported as efficient co-crystal screening methodologies. These techniques include thermal microscopy" " and differential scanning calorimetry methods." ... 

We implemented a high-throughput, parallel approach to enable construct production, expression, and purification of all mutants within a short time, nearly all of which expressed well and were tested in customized, kinase-specific robotic crystallization screens. The methodological improvements implemented here enabled the screening of 44 MK2 constructs, resulting in seven crystal forms, diffraction testing of 500 crystals, and high-resolution data collection and structure determination of-30 MK2/inhibitor complexes. 

Van Eerdenbmgh B, Taylor LS (2010) Small scale screening to determine the ability of different polymers to inhibit dmg crysUillization upon rapid solvent evaporation. Mol Pharm 7 1328—1337 Van Eerdenbmgh B, Taylor LS (2011) An ab initio polymer selection methodology to prevent crystallization in amorphous solid dispersions by application of crystal engineermg principles. Cryst Eng Comm 13 6171-6178... 

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