Crystallins, lens, identification

Udupa, P.E., and Sharma, K.K. (2005) Effect of oxidized betaB3-crystallin peptide (152-166) on thermal aggregation of bovine lens gamma-crystallins identification of peptide interacting sites. Exp. Eye. Res. 80(2), 185-196. 

A Taylor, M Daims, J Lee, T Surgenor (1982) Identification and quantification of leucine aminopeptidase in aged normal and cataractous human lenses and ability of bovine lens LAP to cleave bovine crystallins, Curr Eye Res 2 47-56... 

Identification of Lens Crystallins A Model System for Gene Recruitment... 

Crystal ins. Major water-soluble structural proteins found in fiber cells of vertebrate eye lenses account for the transparency of the lens. Heterogeneous family composed of (our groups a, 0. y, S which have been separated on the basis of size, charge, immunological properties and source. isolation procedures A. Spector. Invest. 

Any regions of the sample under the incident beam (usually a few pm diameter) exhibiting crystallinity, will diffract electrons away from the central spot, forming a diffraction pattern observable at the back focal plane of the objective lens. Like X-ray diffraction, this can provide identification of crystalline phase, orientation, and lattice parameters. In micro-diffraction, the incident beam is focused down to sub-micron areas, but this focusing degrades the diffraction pattern. 

M. Herrmann, J. Klose, H. E. Meyer, Identification of phosphorylation and acetylation sites in aA-crystallin of the eye lens (mus musculus) after two-dimensional gel electrophoresis, Arud. Bioanal. Chem. 376 (2003), 966-972. 

---