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Cross-linking of Ligands

Ro Totfll concsntrstion of bindiriQ prot in[Ro — R + (RL)j Rq is indQpQndsntoft] (RL) Concentration of bound ligand at time t (oo RL) Concentration of bound ligand at t =  [Pg.71]

The slope S has the dimension time-, and kon has the dimension time concentration- (i.e., for example sec- M- ). [Pg.71]

Plotting In (RL)/(RL)j q against t yields a linear form with the slope -koff. [Pg.72]


Blotta, M.H., Marshall, J.D., De Kruyff, R.H. and Umetsu, D.T. (1996) Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a co-stimulatory signal that upregulates IL-4 synthesis. Journal of Immunology 156, 3133-3140. [Pg.366]

Traut, R.R., Casiano, C., and Zecherle, N. (1989) Cross-linking of protein subunits and ligands by the introduction of disulfide bonds. In Protein Function—A Practical Approach (T.E. Creighton, ed.), pp. 101-133. IRL Press at Oxford University, Oxford. [Pg.1122]

The reported application of DSG relates to receptor—ligand studies by covalent cross-linking of their complexes (Waugh et al., 1989). [Pg.222]

Fig. 27 Dendronized salen ligands which were subsequently immobilized by cross-linking of the styryl units... Fig. 27 Dendronized salen ligands which were subsequently immobilized by cross-linking of the styryl units...
Figure 34 Cross-linking of Ni(p,-SCN)2L2 n coordination polymer via carboxyl-carboxyl hydrogen bonds between isonicotinic acid ligands (L) to give a sheet structure which stacks providing channels that can accommodate polycyclic aromatic hydrocarbon guest molecules, here anthracene [80], Oxygen, nitrogen and key hydrogen atoms are shaded nickel thiocyanate polymer and anthracene guest molecules shown in wireframe style. Figure 34 Cross-linking of Ni(p,-SCN)2L2 n coordination polymer via carboxyl-carboxyl hydrogen bonds between isonicotinic acid ligands (L) to give a sheet structure which stacks providing channels that can accommodate polycyclic aromatic hydrocarbon guest molecules, here anthracene [80], Oxygen, nitrogen and key hydrogen atoms are shaded nickel thiocyanate polymer and anthracene guest molecules shown in wireframe style.

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Ligand cross-linking

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