Cross-linking fixation glutaraldehyde

Hassell, J., and Hand, A. (1974) Tissue fixation with diimidoesters as an alternative to aldehydes. I. Comparison of cross-linking and ultrastructure obtained with dimethylsuberimidate and glutaraldehyde./. Histochem. Cytochem. 22, 223-239. 

Other methods for the immobilization of whole cells are fixation on solid supports or matrices by adhesion or adsorption, e.g., on polyurethane foam, direct cross-linking e.g., by glutaraldehyde, or retention of the cells by hollow fibers. The simplest example of the last method is the separation of cells or enzymes from the assay by means of a dialysis lube which allows the exchange of the small substrate and product molecules but not of the larger components. This technique has been highly developed to provide the so-called membrane reactor for the fixation of enzymes. If charged ultrafiltration membranes are used, the cofactor can be retained in its native form316. 

Girardot JM, Girardot MN. Amide cross-linking an alternative to glutaraldehyde fixation. J Heart Valve Dis 1996 5(5) 518-25. 

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