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Cross-linking agents paraformaldehyde

The synthetic procedure for these complexes in acetonitrile with paraformaldehyde as a cross-linking agent is described in more detail in Ref 164. [Pg.109]

To allow efficient extraction of intact mRNA, it is recommended that alcohol fixatives be used in lieu of cross-linking agents such as paraformaldehyde. In our experience protocols that have been developed for studying paraformaldehyde-fixed paraffin-embedded archival specimens in tandem with RNA amplifications produce low yields of RNA from microdissected brain regions and picked cells. Fixation of tissue with methanol or ethanol preserves immu-noreactivity and mRNA in many cases. Accordingly, we immerse... [Pg.222]

See other pages where Cross-linking agents paraformaldehyde is mentioned: [Pg.128]    [Pg.124]    [Pg.56]    [Pg.120]    [Pg.403]    [Pg.175]    [Pg.337]    [Pg.175]    [Pg.19]    [Pg.336]    [Pg.480]    [Pg.654]   
See also in sourсe #XX -- [ Pg.178 ]



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