Cross-linked enzyme aggregates CLEAs

Immobilized enzymes, particularly LAC, have been employed in the degradation of triclosan. The immobilization of LAC from Coriolopsis polyzona through the formation of cross-linked enzyme aggregates (CLEAs) and their subsequent use in an FBR for the removal of endocrine disrupting compounds [42] produced the complete removal of triclosan, nonylphenol, and bisphenol A (5 mg L-1 each) at a HRT of 150 min. The application of CLEAs in a perfusion basket reactor [43]... 

Sheldon, R.A., Sorgedrager, M.J. and Janssen, M.H.A. (2007) Use of cross-linked enzyme aggregates (CLEAs) for performing biotransformations. Chim. Oggi — Chemistry Today, 25, 62. 

Immobilization of Enzymes Cross-linked Enzyme Aggregates (CLEAs) 405... 

Schoevaait R, Wolbers MW, Golubovic M et al. (2004) Preparation, optimization and structures of cross-linked enzyme aggregates (CLEAs). Biotechnol Bioeng 87 754-762 Schuleit M, Luisi PL (2001) Enzyme immobDization in silica-hardened organogels. Biotechnol... 

Yu HW, Chen H, Wang X et al. (2006) Cross-linked enzyme aggregates (CLEAs) with controlled particles appUcation to Candida rugosa liptise. J Mol Catal B Enzym 43(1 ) 124-127... 

Enzyme Crystals and Precipitates. Solid enzyme preparations can be cross-linked with a bifunctional reagent to yield cross-linked enzyme crystals (CLEG) (21), or cross-linked enzyme aggregates (CLEA) (22) that are insoluble in aqueous as well as nonaqueous media. This maintains their catalytic activity and increases their thermostability and resistance to proteases. The activity of the preparation remains high because of the high purity of the protein. CLECs are not commercially available anymore. CLEAs can be dried and suspended in any type of solvent (23). 

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