Cross-bridge disulfide containing

Figure 5.23 SAND can be used to modify amine-containing molecules, and then photo-initiate crosslinking to another amine-containing molecule via a ring-expansion process. The conjugates may be disrupted by reduction of the cross-bridge disulfide with DTT.

Succinimidyloxycarbonyl-a-methyl-a-(2-pyridyldithio)toluene (SMPT) is a heterobifunctional cross-linking agent that contains an amine-reactive NHS ester on one end and a sulfhydryl-reactive pyridyl disulfide group on the other. SMPT is therefore an analog of SPDP that differs only in its cross-bridge which contains an aromatic ring... 

FIGURE 5.17 The hormone insulin consists of two polypeptide chains, A and B, held together by two disulfide cross-bridges (S—S). The A chain has 21 amino acid residues and an intrachain disulfide the B polypeptide contains 30 amino acids. The sequence shown is for bovine insulin. 

Figure 4.4 The reaction of DSP with amine-containing molecules yields amide bond crosslinks. The conjugates may be cleaved by reduction of the disulfide bond in the cross-bridge with DTT.

Crossed-linked polymers contain disulfide bridges or branches that connect neighboring chains. 

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