Coupling agents metabolites

A method for the detection of nerve agent metabolites ba,sed on GC coupled with an atomic emission detector (AED) has been reported (Creasy etal., 1995). The nerve agent degradation products were extracted from spiked water, wipes, and soil samples. The extracted samples were deriva-tized with 1% trimethylchlorosilane in bis-(trimethylsilyl) trifluoroacelamidc. The GC/AED technique was used for separation, detection, and determination of OP nerve agent metabolites. 

Only the metabolites listed in Table I as coupling agents need be considered at this point others will be discussed in later sections, or in a monograph in preparation. The coupling ratio between ATP regeneration and DPNH oxidation is, of course, the familiar P-O ratio, and a value of 3 is well established for typical eukaryotic cells. The tabulated value of 4 for TPNH may require comment. At least in many types of cells, the TPN system seems to be always far more reduced than the DPN system. In an early review of metabolic functions of... 

K.-M. Chu, S.-M. Sliieh, S.-H. Wu and O. Y.-P. Hu, Enantiomeric separation of a cardiotonic agent pimobendan and its major active metabolite, UD-CG 212 BS, by coupled achiral-cliiral normal-phase high-performance liquid chromatography , 7. Chromatogr. Sci 30 171-176(1992). 

Oswald EO, Albro PW, McKinney JD. 1974. Utilization of gas-liquid chromatography coupled with chemical ionization and electron impact mass spectrometry for the investigation of potentially hazardous environmental agents and their metabolites. J Chromatogr 98(2) 363-448. 

Employing the MesSiCl/col couple as titanocene-regenerating agent, we have prepared both monocyclic sesquiterpenoids, such as the metabolite 19 found in the fragrant plant Artemisia chamaemelifolia (Scheme 24), and monocyclic triterpenoids, such as achilleol A (9), starting from epoxygeranylacetone derivative 8 and using only substoichiometric proportions (0.2 equiv) of the titanocene catalyst [53]. 

Oswald, E.O., P.W. Albro, and J.D. McKinney, Utilization of Gas Liquid Chromatography Coupled with Chemical Ionization and Electron Impact Mass Spectrometry for the Analysis and Characterization of Potentially Hazardous Environmental Agents and their Metabolites. J. Chromatog. 363 (1974). 

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