Corynebacterium strain

Many aldehyde reductases transform both aldehydes and ketones. For example, phenylacetaldehyde reductase (PAR) from a styrene-assimilating Corynebacterium strain, ST-10, reduces hexyl aldehyde and phenylacetaldehyde [22aj. Other aldehyde reductases such as one from Sporobolomyces salmonicolor also reduce aldehydes as well as ketones [22b]. 

Figure 8.35 Reduction of ketones with phenylacetaldehyde reductase from Corynebacterium strain ST-10 [26].

Itoh N, R Morihama, J Wang, K Okada, N Mizngnchi (1997) Pnrification and characterization of phenylacetaldehyde reductase from a styrene-assimilating Corynebacterium strain, ST-10. Appl Environ Microbiol 63 3783-3788. 

Table 15-18. Examples of substrates of phenylacetaldehyde reductase from Corynebacterium strain, ST-10138.

Based on this innovative two-step fermentation process, Anderson et al. (1985) has expressed a 2,5-DKG reductase from Corynebacterium ATCC 31090 into the Erwinia herbicola ATCC 21988 with the expression vector ptrpl-35. The final recombinant E. herbicola strain could produce 1 g/L of 2-KLG from saturated D-glucose solution. By the protoplast fusion, Lin et al. (1999) fused an Erwinia herbicola and a Corynebacterium strain. The resultant strain could produce 2.07 g/L of 2-KLG (Fig. 12.5). 

This approach has been used with a wide variety of organisms, although it is more commonly employed using Arthrobacter simplex, Brevibacterium lipolyticum, Corynebacterium spp and certain strains of Nocardia. 

Omori T, L Monna, Y Saiki, T Kodama (1992) Desulfurization of dibenzothiophene by Corynebacterium sp. strain SYl. Appl Environ Microbiol 58 911-915. 

Hydroxylation of n-octane by cell extracts of Gordonia (Corynebacterium) sp. strain 7E1C (Cardini and Jurtshuk 1968), and of some strains of Acinetobacter calcoaceticus induced with -hexadecane (Asperger et al. 1981). 

Cardini G, P Jurtshuk (1968) Cytochrome P-450 involvement in the oxidation of -octane hy cell-free extracts of Corynebacterium sp. strain 7E1C. J Biol Chem 243 6070-6072. 

Nakamura T, T Nagasawa, F Yu, I Watanabe, H Yamada (1994b) Purification and characterization of two epoxide hydrolases from Corynebacterium sp. strain N-1074. Appl Environ Microbiol 60 4630-4633. 

An additional variant that involves both hydrolytic and reductive reactions has been found in the degradation of 2,4-dichlorobenzoate by Alcaligenes denitrificans strain NTB-1 (van den Tweel et al. 1987) (Figure 9.16), and via the CoA ester by Corynebacterium sepedonicum (Romanov and Hausinger 1996). 

Romanov V, RP Hausinger (1996) NADPH-dependent reductive ortho dehalogenation of 2,4-dichlorobenzoic acid in Corynebacterium sepedonicum KZ-4 and coryneform bacterium strain NTB-1 via 2,4-dichlo-robenzoyl coenzyme A. J Bacteriol 178 2656-2661. 

The Japanese cooperating organizations also disclosed several other microorganisms in their awarded patents. The reports include strains of Corynebacterium (T09 and T04 strains) [115], Gordona [116], Bacillus [117], Sphingomonas, Agrobacterium, and Arthrobacter [118]. 

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