Coproduction of 3-HP and PDO From Glycerol

pneumoniae, 3-HP and PDO are derived from the same intermediate, 3-HPA. The fate of 3-HPA depends on the activity of relevant enzymes (oxidoreductases and 3-HPA-specific ALDH) and the availability of redox cofactors (NAD and NADH). If a single product, either 3-HP or PDO, should be produced at high yield, one of the other types of enzymes should be disrupted and one kind of cofactor should be regenerated efficiently. When compared to gene disruptions, cofactor regeneration is much more challenging. In K. pneumoniae, cofactor regeneration is performed by oxidative 

Although Ashok et al. (2011) and Huang et al. (2012) reported successful coproduction of 3-HP and PDO from glycerol by growing cells of K. pneumoniae, a significant amount of organic acids and alcohols were produced as by-products. To avoid 

MICROBIAL PRODUCTION OF 3-HYDROXYPROPIONIC ACID FROM RENEWABLE SOURCES 

---