Copper I as the Neo-Cuproin Complex

All these assay methods shall be discussed in the following sections  

Materials Required hydroxyammonium chloride solution (10% w/v) 25 ml sodium citrate solution (30% w/v) 50 ml ammonia solution neo-cuproin solution (0.1% w/v in absolute ethanol) 50 ml chloroform  

DETERMINATION OF IRON (III) AS THE 8-HYDROXY QUINOLATE COMPLEX [IRON (III) OXINATE] 

Theory Iron (III) upto an extent of 50-200 meg can be extracted effectively from an aqueous solution with a 1% solution of 8-hydroxyquinoline (symbolized as HQ) in chloroform by carrying out a double extraction when the pH of the resulting aqueous solution ranges between 2 and 10. Evidently, between pH 2.0 to 2.5 metals like Ni, Co, Ce (III) and A1 do not interfere at all. However, iron (III) oxinate is dark-coloured in chloroform and absorbs at 470 nm. 

Materials Required Hydrated ammonium iron (III) sulphate 0.0266 g oxine solution ( AnalaR -Grade, 1% w/v in chloroform) 50 ml chloroform 100 ml  

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