CooH gene

The amino acid sequence of the hormone is (NII2)-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-(COOH). Using the genetic code, it is straightforward to deduce an appropriate DNA sequence that would code for this polypeptide. For production of the hormone in E. coli, the gene fusion method was used. 

The widely accepted model of GPCR homologous desensitisation describes a system in which the agonist-activated receptors couple to G proteins to induce a cellular response, and are subsequently phosphorylated by members of GRK, with GRKs invariably phosphorylating serine or threonine residues on the third intracellular loop or COOH terminus of the receptors (Reiter and Lefkowitz 2006). The GRK family consists of seven different genes. 

It is obvious that it is prudent to check the correctness of the amino acid sequence derived from the base sequence of the gene not only at the NHg and COOH termini, which is the common practice, but throughout the entire protein. This would help to uncover any significant errors as well as address the possibility of post-translational modifications. 

Marr, R.S., Blair, L.C., aud Thoruer, J. (1990). Saccharomyces cerevisiae STEM gene is required for COOH-terminal methylation of a-factor mating pheromone. J Biol Chem 265 20057-20060. 

He, B., Lee, L.W., Minges, J.T. and Wilson, E.M. (2002) Dependence of selective gene activation on the androgen receptor NH2-and COOH-terminal interaction. The Journal of Biological Chemistry, XJ7, 25631-25639. 

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