Containing bacteriorhodopsin

Fig. 8.19 SEM images of mesolamellar thin films produced by intercalation of nanosheets of (A) aminopropyl-functionalized silica or (B) AMP between stacked purple membrane fragments containing bacteriorhodopsin (scale bars= 10pm).

Figure 23-5 Resonance Raman spectra. (A) Of the retinaldehyde-containing bacteriorhodopsin bR568 (see Fig. 23-45) and its 12,14-2H and 14-13C isotopic derivatives. (B) Of bRS68 labeled with the dominant internal coordinates that contribute to the normal modes. From Lugtenburg et al 7...

An artificial system was created to elegantly demonstrate the basic principle of the chemiosmotic hypothesis. Synthetic vesicles containing bacteriorhodopsin, a purple-membrane protein from halobacteria that pumps protons when illuminated, and mitochondrial ATP synthase purified from beef heart were created (Figure 18.26). When the vesicles were exposed to light, ATP was formed. This key experiment clearly showed that the respiratory chain and ATP synthase are biochemically separate systems, linked only by a proton-motive force. 

Figure 18.26. Testing the Chemiosmotic Hypothesis. ATP is synthesized when reconstituted membrane vesicles containing bacteriorhodopsin (a light-driven proton pump) and ATP synthase are illuminated. The orientation of ATP synthase in this reconstituted membrane is the reverse of that in the mitochondrion.

Baateriorhodopsin-containing Membrane. The preparation of BLM containing bacteriorhodopsin was first reported by Dancshazy and Katvaly (57) and Herrmann and Hayfield These membranes... 

Ray field G W 1994 Photodiodes based on bacteriorhodopsin Molecular and Biomolecular Electronics (Advances in Chemistry Series 240) ed R R Birge (Washington, DC American Chemical Society) pp 561-75 Tanabe K, Hikuma M, SooMi L, Iwasaki Y, Tamiya E and Karube I 1989 Photoresponse of a reconstituted membrane containing bacteriorhodopsin observed by using an ion-selective field effect transistor J. Biotechnol. 10 127-34... 

Electrochemical Processes in Membranes That Contain Bacteriorhodopsin... 

Supramolecular biological assemblies, like virus [52] or membrane fragments [261,262] containing bacteriorhodopsin have also been inserted in multilayers built by ESA. 

Hampp, N., Popp, A., Brauchle, C., and Oesterhelt, D., Diffraction efficiency of bacteriorhodopsin films for holography containing bacteriorhodopsin wildtype BRwt and its variants BR jg and BRd96n.> / Phys. Chem., 96, 4679—4685,1992. 

Hellingwerf KJ (1979) Structural and functional studies on lipid vesicles containing bacteriorhodopsin. PhD Thesis, University of Amsterdam. 

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