Conservative regions

A sequence alignment establishes the correspondences between the amino adds in th unknown protein and the template protein (or proteins) from wliich it will be built. Th three-dimensional structures of two or more related proteins are conveniently divided int structurally conserved regions (SCRs) and structurally variable regions (SVRs). Ihe structural conserved regions correspond to those stretches of maximum sequence identity or sequenc... 

Backbone generation is the first step in building a three-dimensional model of the protein. First, it is necessary to find structurally conserved regions (SCR) in the backbone. Next, place them in space with an orientation and conformation best matching those of the template. Single amino acid exchanges are assumed not to affect the tertiary structure. This often results in having sections of the model compound that are unconnected. 

SCR (structurally conserved regions) sections of a biopolymer sequence that are identical to that of another sequence, for which there is a known three-dimensional structure... 

Approximately 500 of the 820 amino acid residues of the myosin head are highly conserved between various species. One conserved region, located approximately at residues 170 to 214, constitutes part of the ATP-binding site. Whereas many ATP-binding proteins and enzymes employ a /3-sheet-a-helix-/3-sheet motif, this region of myosin forms a related a-f3-a structure, beginning with an Arg at (approximately) residue 192. The /3-sheet in this region of all myosins includes the amino acid sequence... 

N-terminal domain is the least conserved region and contains the transactivation domain AF-1. 

The universal antibiotic pactamycin targets a highly conserved region of 16S rRNA, contacting the tips of helices 23b and 24a in the central domain. Pactamycin folds up to mimic a RNA dinucleotide in that its... 

HIGHLY CONSERVED REGIONS IN THE SMALL CODH SUBUNIT... 

Rifampicin is the semisynthetic derivative used widely in the UK. Resistance to rifampicin is primarily due to chromosomal mutations resulting in an altered RNA polymerase which is less well inhibited by the drug. The mutations tend to be clustered within short conserved regions of the J3 subunit gene of RNA polymerase. Similar mutations have been found in all bacterial species studied thus far. 

Fig. 1.1 ATP bound to cAMP protein kinase and a schematic representation indicating the non-conserved regions (hydrophobic pocket and specificity surface) of the pocket utilised in the development of protein kinase inhibitors.

Edwards TG, Wyss S, Reeves JD, et al. Truncation of the cytoplasmic domain induces exposure of conserved regions in the ectodomain of human immunodeficiency virus type 1 envelope protein. J Virol 2002 76(6) 2683-2691. 

Probes that hybridize to the target and also to either preamplifier or amplifier molecules are termed label extenders. The locations of the capture and label extender probes used in the hepatitis B virus (HBV), hepatitis C virus (HCV), and HIV-1 assays are shown in Figs. 3,4, and 5, respectively. All target probes are designed to hybridize to the most conserved regions of the genomes. For HBV, the... 

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