Conjugation oligo

Recent progress in research on anion-responsive TC-conjugated oligo-pyrroles 13CC4100. 

Transition from tunneling to hopping transport in long, conjugated Oligo-imine wires connected to metals. 

The classical catalyst system Re207/Al203/Bu Sn has been used to achieve the ADMET polymerization of various divinylsilanes to produce a-jt-conjugated oligo(silylene-vinylene)s [162]. These compounds could not be polymerized with Schrock s, Grubbs, and the WClg/SnBu classical catalysts. 

Sato C, Kitajima K, Inoue S, Seki T, Troy FA II, Inoue Y (1995) Characterization of the antigenic specificity of four different anti-(a2,8-linked polysialic acid) antibodies using lipid-conjugated oligo/polysialic acids. J Biol Chem 270 8923-18928... 

Figure 2. Photo-cross-linking reaction of psoralen-conjugated oligo(nucleoside phosphoro-thioate) (PS-P-As) with its complementary oligoribonucleotide (sense-RNA). [PS-P-As] = [sense-RNA] = 20pM. UVA-irradiation was carried out in phosphate buffered saline (0.135M NaCl, pH7.0) at 365nm with l.lmJ/cm sec.

Figure 3. Effect of psoralen-conjugated oligo(nucleoside phosphorothioate)s (PS-S-Oligo) on the proliferation of human cervical carcinoma cell, C4II. and SiHa. [A] C4II cell line. PS-P-As without UVA-irradiation (4-), PS-P-scr without UVA-irradiation (A ), PS-P-As with UVA-irradiation ( ), PS-P-scr with UVA-irradiation (A). [B] SiHa cell line. PS-P-As without UVA-irradiation ( ), PS-P-scr without UVA-irradiation (A), PS-P-As with UVA-irradiation ( ), PS-P-scr with UVA-irradiation (A). UVA-irradiation 365nm, I.lmJ/cm sec. Cell growth was evaluated by modified MTT assay.

A large part of organic and macromolecular chemistry starts with the chemical functionalization of benzene, and benzene units serve us building blocks for important polymers. Naturally, benzene-based aromatic materials also represent an important subclass of jt-conjugaled architectures. Despite some synthetic difficulties related to the generation of structurally well-defined oligo- and poly(phenyl-... 

In conclusion, a variety of linear or cyclic oligo(phospholes)s and their derivatives are accessible via a set of efficient synthetic strategies. The potential of these compounds as advanced 71-conjugated systems is broadened by the presence of reactive trivalent P-centres, which allow a range of additional chemical modifications to be achieved. However, elucidation of structure-property relationships for these derivatives is still needed. 

Polymeric (isocyanide)gold(i) aryls ( gold oligo-phenylene-ethynylene-isonitriles ) were tested as electrical conductors at metal-molecule-metal junctions (7r-conjugated molecular wires), but the preparation, structure, and properties of the materials were not fully disclosed (Scheme 52).218... 

If an individual nucleotide is modified in the appropriate way, various enzymatic techniques can be used to polymerize the derivative into an existing oligonucleotide molecule. Alternatively, nucleotide polymers can be treated with chemical activators that can facilitate the attachment of a label at particular reactive sites. Thus, there are two main approaches to modifying DNA or RNA molecules enzymatic or chemical. Both procedures can produce highly active conjugates for sensitive assays to quantify or localize the binding of an oligo probe to its complementary strand in a complex mixture. 

The chemical modification of nucleic acids at specific sites within individual nucleotides or within oligonucleotides allows various labels to be incorporated into DNA or RNA probes. This labeling process can produce conjugates having sensitive detection properties for the localization or quantification of oligo binding to a complementary strand using hybridization assays. 

Some form of chemical labeling process must be used regardless of whether the final oligo conjugate is created by enzymatic or strictly chemical means. If enzymatic modification is to be done, the initial label still must be incorporated into an individual nucleoside triphosphate, which then is polymerized into an existing oligonucleotide strand (Section 1, this chapter). Fortunately, many useful modified nucleoside triphosphates are now available from commercial sources, often eliminating the need for custom derivatization of individual nucleotides. 

Purify the hydrazide-labeled oligo by gel filtration on desalting resin using 10 mM sodium phosphate, 0.15M NaCl, lOmM EDTA, pH 7.2. The probe now may be used to conjugate with an aldehyde-containing molecule. 

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