Confocal scanning laser microscopy antibody labeling

Transmission electron microscopy analyses of ultrathin sections have indicated that there are several vacuoles in myrosin cells of embryos. These vacuoles contain myrosinase, as revealed by immunogold labelling studies, and have been referred to as myrosin grains.40, 1 Recently, confocal laser scanning immuno-microscopy analysis with the anti-myrosinase monoclonal antibody 3D7 has been... 

Confocal laser scanning microscopy can be used in conjunction with microwave heating for examining the three-dimensional structure and cellular interrelationships in sections of paraffin-embedded tissues (Boon and Kok, 1994). Tissues are fixed with Kryofix, a coagulant fixative containing 50% ethyl alcohol and polyethylene glycol (PEG molecular weight 300) for 90 sec in a microwave oven. The use of thick paraffin sections (15 (xm) and fluorescently labeled antibodies is preferred. 

Fig. 1. Double immunofluorescence detection of cytoplasmic cytokeratin 19 intermediate filaments (CK19) (FITC, green/ filamentous) and the cell-membrane associated connexin 43 (Cx43) gap junction protein (Cy3, red/dot-like) in the stratified squamous epithelium of human cervix. Combination of mouse monoclonal (CK19) and rabbit polyclonal (Cx43) antibodies and distinct fluorochrome-labeled secondary antibodis. Confocal laser scanning microscopy, a projection of five optical layers.

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