Concanavalin manganese

Concanavalin, 2, 773 Concanavalin A, 6,572 manganese, 6,587 Concentration mineral processing flotation, 6,780 Concerted electron transfer oxidases, 6,683... 

The NMRD profiles of manganese(II) aqua ion and of manganese(II)-concanavalin A have already been analyzed in Section I.C.3. The NMRD... 

At variance with the manganese(II) concanavalin A, the relaxivity of which increases with increasing temperature at low field, indicating the... 

To immobilize a protein the biologic activity of which depends on a cofactor, this cofactor has to be present in sufficient concentration during the immobilization step. Thus, for example, the lectin Concanavalin A is immobilized in the presence of a buffer containing 0.1 M glucose or a-methylmannoside, 1 mM calcium chloride and 1 mM manganese chloride. 

For additional references of early work on lectins see Lis and Sharon (ref. 2). t Abbreviations Con A, concanavalin A (which refers to the protein with a full complement of manganese and calcium, i.e., the holoprotein) aMeMan, methyl -D-mannopyranoside aMeGlc, methyl a-D-glucopyranoside /JIphGlc, /J-(O-iodophenyl) D-glucopyranoside 0IphGal,... 

Figure 16.5 Affinity chromatography of peroxidase (after A. Borchert, P.O. Larsson and K. Mosbach, J. Chromatogr., 244, 49 (1982)]. Conditions sample, 4.1 ml of solution with 1 pg ml of protein column, 5cm x 5 mm i.d. stationary phase, concanavalin A on LiChrospher Si 1000, 10pm mobile phase, 0.05M sodium acetate, 0.5 M sodium chloride, 1 mM calcium chloride, 1 mM manganese chloride, pH 5.1 t shows pulse of 4.1 ml of 25 mM a-methyl-o-glycoside detector, solid line, UV, 280 nm broken line, visible, 405nm. Peaks = nonretained proteins this peak contains less than 2% peroxidase 2 = peroxidase.

Fig. 2. Structure of the manganese and calcium dimer in concanavalin A, based on (14, 15, 18,22).

An interesting point is that manganese, as well as other divalent transition metals, plays an essential role in saccharide binding in concanavalin A, a member of the plant proteins which are also known as hemagglutinins or plant lectins. Concanavalin A occurs in quantities of > 5% DM in legumes (e.g., the Jack bean, Canavalia ensi-formis). The function of plant lectins in saccharide binding sites is unknown, but a defense mechanism may be involved (Kalb (Gilboa) et al. 2000). 

Manganese(II) in Concanavalin A and Other Lectin Proteins A. Joseph Kalb(Gilboa), Jarjis Habash, Nicola S. Hunter,... 

Manikandan P, Carmieli R, Shane T, Kalb AJ, Goldfarb D. 2000. W-band ENDOR investigation of the manganese-binding site of concanavalin A determination of proton hyperfine couplings and their signs. JAm Chem Soc 122 3488-3494. 

---