Con A agarose

Dextran B-1355-S was adsorbed to three different forms of immobilized con A (con A-agarose,379 poly(L-leucyl)-con A,379 and... 

Concanavalin A-agarose has been used in the purification of the glycoprotein hormones, chorionic gonadotropin, and follicle stimulating and leuteinizing hormones [129]. Delipidated rat brain glycoproteins in 1% sodium deoxycholate can be purified on Con A-agarose [130]. 

Murthy and Herez described a procedure for the purification of a-antitrypsin based on affinity chromatography on Con A-Sepharose [131]. The glycoprotein a,-fetoprotein has been purified on Con A-agarose [132]. Affinity chromatography of a,-fetoprotein on Con A-agarose has been used to demonstrate two molecular variants of the protein and was used in the resolution of these two forms [133]. 

Fig. 1. Templates for the crossed immunoaffinoelectrophoresis technique, (a) first dimension (b) second dimension.- strip to be removed and replaced by 1.1 mL of agarose -f 200 pL of Con A sdutiwi /// agarose containing antiserum paper...

ReBll the resultant trough wth 1.1 mL of agarose (kept in the 50-56°C water bath) to which has just been added the 200 pL of Con A solution. 

After gelling, punch out a well in the Con A containing-agarose as indicated in Fig. la. 

It is Important to preheat the tubes containing the Con A solution before adding the 1.1 mL of agarose, otherwise, agarose will gel in the tube. 

Conoanavalin A (Con A) Mn"+ Ca + 4% Agarose (Sigma) SpectraGel (Spectrum) Sepharose (Pharmacia) 0,1-0.2 M a-D-methylmannoside, 10 mM a-D-methylglucoside Buffer must not contain EDTA... 

Con A Sepharose 4B 4% agarose Concanavalin A 10-16mg/mL 4-9 45-165 Immunoglobulins, hormone receptors, polysaccharides, glycoproteins, membrane proteins, lipoproteins AP Biotech ... 

Figure 12.20 Process of immunoelectrophoresis (A) Chamber uMh pattern cut in agarose/agar. (B) Round wells JUled with antigens Bovine serum albumin (BSAJ and Bovine serum (BS). Electrophoresis separates the sample into different con uiuls. (Cl Addition of antiserum (mbit anti-BS and incubation leads to dyfu-Sion qf antigens and antibody to give precipitin bands.

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