Components in pyrolysates from lysine, leucine, and tryptophan

Components in Pyrolysates from Lysine, Leucine, and Tryptophan (2902) 

Schlotzhauer (722)]. The results reported by Higman et al. are summarized in Table XXV-18. 

Tryptophan was fonnd to be the precursor in tobacco of harman (l-methyl-9//-pyrido[3,4- ]indole) and norharman (9H-pyrido[3,4-fc]indole) in tobacco smoke, componnds originally identified in tobacco and tobacco smoke by Poindexter and Carpenter (2972). That tryptophan was indeed a precursor in tobacco of the barmans in smoke was demonstrated by addition of radiolabeled tryptophan to tobacco and identihca-tion of radiolabeled harman and norharman in the MSS. 

More recent amino acid pyrolysis studies led to the isolation and identification of several 7V-heterocyclic amines reported not only to be tnmorigenic to mouse skin bnt also to show inordinately high mntagenicity [Ames bioassay 

In 1977, Sugimura et al. (3829) reported the identification of the potent mutagens 3-amino-l-methyl-5//-pyrido[4,3-/ ] indole (Trp-P-2) and 3-amino-l,4-dimethyl-5//-pyrido[4,3-/ ] indole (Trp-P-1) in tryptophan pyrolysate. The next year, Yamamota et al. (4365a) identified two potent mutagens in glutamic acid pyrolysate aminodipyrido[l,2-a 3 ,2 -d]imida-zole (Glu-P-2) and 2-amino-6-methyldipyrido[l,2-a 3 ,2 -t/] imidazole (Glu-P-1). 

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