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Competitive RIA of Free Anti-Insulin Antibodies with PEG Precipitation Semi-Quantitative Assay

Competitive RIA of Free Anti-Insulin Antibodies with PEG Precipitation (Semi-Quantitative Assay) [Pg.648]

PURPOSE AND RATIONALE Anti-insulin antibodies (ALA) develop in the serum of many patients who are receiving insulin treatment (Berson et al 1956). In addition, insulin auto antibodies (IAA) are detected in insulin dependent diabetes melhtus (IDDM) or Type I Diabetes before insulin therapy (Palmer et al 1986). [Pg.648]

The determination of circulating anti-insulin antibodies is of clinical importance for the following reasons  [Pg.648]

presence of free anti-insulin antibodies in serum interferes with the determination of insulin by immunoassays (Kuzuya et al. 1977) [Pg.648]

high titers of ALA may induce a state of insulin re-sistence [Pg.648]




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Anti antibodies

Antibody anti-insulin

Antibody, insulin

Competition assay

Competition with

Free precipitation

Insulin antibodies assay

Insulin assay

Insulin precipitation

PEG-antibody

Precipitating antibodies

Quantitative precipitation assay

RIAs

Semi-quantitative

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