Combined with Bismarck Brown

3 Coomassie Brilliant Blue R250 Combined with Bismarck Brown R 

Gels are stained very sensitive and quick by combination of Coomassie Brilliant Blue R250 with Bismarck Brown R (Basic Brown4 C.1.21010). In our opinion, this is the best staining method using organic dyes. 

2 g Coomassie Brilliant Blue R250 are dissolved in 40 ml methanol or non-denaturated ethanol then 10 ml glacial acetic acid are added and the solution is filled up to 100 ml with deionized water. Stable at RT B 0.05 g Bismarck Brown R are dissolved in 40 ml methanol or non-denaturated ethanol then 10 ml glacial acetic acid are added and the solution is filled up to 100 ml with deionized water. Stable at RT. 

D destaining solution 10% acetic acid (v/v), 30% methanol or non-denaturated ethanol or isopropanol (v/v) in deionized water 

The gel is fixed for 5-10 min in Soln. D after electrophoresis and stained with Soln. C (about 20 ml for a 8 x 6 x 0.1 cm gel) for 20 min with agitation. Flocculation of dye does not influence staining. 

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