Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Column preparation film formation

Gas Chromatography-Mass Spectrometry. Prepare methoxime derivatives by heating the extract from the thin-layer chromatographic plate with 100 xl of an 8% solution of methoxyamine hydrochloride in dry pyridine at 60° for 30 minutes, and evaporate in a rotary film evaporator. Silylate by dissolving the residue in 100 Lll of chloroform, adding 100 Lll of A,0-bis(trimethylsilyl)acetamide and 20 Lll of trimethylchlorosilane, then seal, and heat for 2 hours at 60°. When only 5a-estrane-3P,17a-diol is to be confirmed the methoxime formation can be omitted. Prepare a 2-cm column of Sephadex LH-20 in a Pasteur pipette with a cotton wool plug, using a... [Pg.95]


See other pages where Column preparation film formation is mentioned: [Pg.75]    [Pg.598]    [Pg.599]    [Pg.212]    [Pg.212]    [Pg.95]    [Pg.97]    [Pg.36]    [Pg.266]    [Pg.393]    [Pg.410]    [Pg.29]    [Pg.717]    [Pg.1386]    [Pg.3310]    [Pg.37]    [Pg.377]    [Pg.1533]    [Pg.168]    [Pg.94]    [Pg.148]    [Pg.669]    [Pg.36]    [Pg.390]    [Pg.367]    [Pg.58]    [Pg.496]    [Pg.1806]    [Pg.1819]    [Pg.2255]    [Pg.255]    [Pg.39]    [Pg.1461]    [Pg.468]   
See also in sourсe #XX -- [ Pg.145 ]




SEARCH



Column preparation

Column preparative

Film format

Film formation

Film preparation

© 2024 chempedia.info