Column chromatography eluent delivery

Eluent delivery was provided by two model 510 high-pressure pumps coupled with automated gradient controller model 680 (Waters Chromatography Division, Millipore, MA, USA) and a model 7125 infection valve with a 20-(il loop from Rheodyne (Cotati, CA, USA) Stainless-steel columns (30 x 0.40 cm I.D.) packed from Tracer Analltica (Barcelona, Spain) with 10 un particle diameter Spherisorb ODS-2 (Merck, Darmstadt, FRG) were used. Four different LC mobile phase compositions were tested acetonitrile-water... 

Sugars. The high performance liquid chromatography (HPLC) method described by Hunt et al. (8) was used to determine the sugars in the kiwifruit and nectars with some modifications (21). A Waters Association Chromatograph equipped with a Model 6000-A solvent delivery system, a Model R401 refractometer detector, a D6K universal injector column was a 30 cm x 4 mm i.d. stainless steel tube packed with u-bondapak-carbohydrate (Waters Associates). The precolumn was packed with CO-PELL PAC (Whatman). The eluent was acetonitril and distilled water (85/15, v/v). 

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