Chromatographic analysis column

Mannanase. Sprucewood delignified at room temperature was treated with mannanase as described by Yamazaki (15). However, a higher mannanase concentration was used, and the reaction was followed by means of quantitative, column chromatographic analysis of the reducing sugars in the reaction solutions. 

The Pittsburgh and Midway product from Merriam, Kansas, 122, is quite similar to the Tacoma SRC product, 308. The amount of solvent residue is very low. The solvent in both cases is of moderate molecular weight, and there appears to be very little breakdown of large molecules in the coal to produce either volatile compounds that would be left as residue in the product or to produce aromatic compounds that could be eluted in the column chromatographic analysis. 

Most small peptides derived from protein sources contain a variety of polar and non-polar amino acids and as such are more difficult to handle. Nevertheless appreciation of the problems and considered chemical manipulation have allowed their MS elucidation [177-184] although a practical limit of about six amino acids in the peptide is reached before degradative procedures become advisable. An important step before sequencing a peptide by mass spectrometry is whenever possible to obtain the amino acid content by hydrolysis and conventional column chromatographic analysis. This assists in the selection of any chemical pretreatment and with the spectral interpretation. A variety of small peptides has been identified by a combination of methods and include 5 - 0X0 - L - prolyl - L - histadyl - L - prolinamide (2-pyrollidone-5-carboxylyl-... 

Figure 4. Dowex-l-X8 borate anion-exchange column chromatographic analysis of supernatants from 24 hour incubations of P. putida with 4-deoxy-4-fluoro-D-(U- C)glucose. 0 Radiolabeled metabolites internal non-radiolabeled carbohydrate standards. All compounds were eluted with a linear gradient of ammonium tetraborate.

Figure 8. BioGel A 1.5m column chromatographic analysis of SDS-solubilized cell envelope isolated from P. nutida after 24 hour incubation with 4-deoxy-...

Table II. Primary Condensing Unit Pyrolytic Oils and the Yield of Various Classes of Compounds Obtained by Silica-Gel Column Chromatographic Analysis (a)...

Berndge B J, Chao W, R., and Peters J H. (1971) Column chromatographic analysis of tryptophan with the basic amino acids Anal Biochem 41, 25 264. 

The hen liver nuclei contain both ADP-ribosyltransferase and poly(ADP-ribose) synthetase. To separate the ADP-ribosyltransferase from poly(ADP-ribose) synthetase, the 0.6 M potassium chloride extract from the nuclei was applied to a Sephadex G-200 column and eluted with the 0.1 Af Tris-buffer, pH 8.0. Each fraction was incubated with 1 vaM [adenine- H]NAD and 100 jug of whole histones, in a total volume of 0.2 ml containing 50 mAf Tris-Cl" buffer, pH 9.0, and the radioactivity in the acid-insoluble fraction was determined. The result shows the two fractions containing the enzyme activities which catalyze the incorporation of the ADP-ribose moiety from NAD to the whole histones. From the hydroxyapatite column chromatographic analysis of the products formed by the respective fraction, we found that the former fraction contains poly(ADP-ribose) synthetase and the latter fraction contains poly-... 

Wadman, S.K., De Bree, PK., Van der Heinden, C. and Van Sprang, F.J. (1971) Automatic column chromatographic analysis of urinary and serum imidazoles in patients with histidinaemia and normals. Clin. Chim. Acta, 31 215. 

This book comprises 12 chapters covering an introduction, porous adsorbents, adsorption equilibrium, diffusion in porous particles, kinetics of adsorption in a vessel, kinetics of adsorption in a column (chromatographic analysis and breakthrough curves), heat effects, regeneration of spent adsorbent, chromatographic separation, pressure swing adsorption and adsorption for energy transport. The text comprises a blend of mathematical analysis and descriptions of plant and processes. Each chapter is fully referenced. 

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