Column, capillary weight, limits

With decreasing packing size in SEC columns, the probability of physical entrapment of macromolecules increases. To estimate the molecular weight limit above which ultrafiltration will occur, we must first calculate an average radius of the interstices formed in a packed bed. This is done by assuming that the packed column consists of a bundle of capillaries in which the capillary radius can be estimated from the bed hydraulic radius ... 

---