Collagen solution preparation

To prepare sandwich-cultured hepatocytes, neutralized collagen solution (0.1 ml, 1.5 mg/ml, pH 7.4) is added to the monolayers 24 h after the cells are seeded. Cultures with collagen overlay are incubated for 45 min at 37 °C in a humidified incubator to allow the collagen to gel before addition of DMEM. Medium is changed on a daily basis until the fifth day after the cells are seeded. These hepatocytes are referred to as day 5 or long-termed cultured hepatocytes. 

Continue embedding the explants in this manner, changing the collagen solution in which the explants are washed every three dishes prepared (tee Note 7). 

Figure 13 shows the noise conductivity dispersion curve of a collagen solution (methanol+ 5% water, 10 mol/literHCl) prepared following the procedure proposed by Herbage. A sigmoidal conductivity curve is obtained when the solvent noise is substracted from the total measured noise conductivity. 

Boki et al. reported the structural analysis of alkali-treated [4] collagen fibers followed by preparation in acidic pH solution [17]. The cumulative pore size distributions are shown in Fig. 12. No micropores with radii less than 2.0 nm are found on the raw collagen fibers prepared from steer hide. However, micropores with radii of 1.2 nm or larger appear after the alkali treatment. These results are not in agreement with the data obtained by the moisture adsorption method [3] or low-angle X-ray diffraction [11]. 

Chitosan/ collagen Membranes were prepared by solvent evaporation of chitosan and collagen solution. Controlled release of propi anolol hydrocliloride. Tacharodi and Rao, 1995... 

Viscoelastic collagen solution for opthalmic use and method for preparation 4,713,446 1987 Devore et al. 

Type I collagen can be prepared relatively easily in a large quantity and it is also commercially available. As a result, cell cultures with this collagen are common Indeed, this gel culture is widely used to study cancer cell transferability. The culture is made by first preparing a gel that contains a culture base and the cell is planted atop the gel. If cells are suspended in a collagen solution and the solution is geUed, a containing gel can be prepared [4]. 

Collagen. Solutions of collagen may be prepared by extraction of tendon with dilute acetic acid. The anomalous viscosity and double refraction of flow of such a solution indicate the presence of very elongated molecules (Mehl, 1939). When a collagen solution is dialyzed in the absence of salt, bringing the pH above 4, a gel is formed. Addition of a very small amount of salt converts the gel to a precipitate (Bessey, 1939). 

M.p. 190-192 C. The enolic form of 3-oxo-L-gulofuranolactone. It can be prepared by synthesis from glucose, or extracted from plant sources such as rose hips, blackcurrants or citrus fruits. Easily oxidized. It is essential for the formation of collagen and intercellular material, bone and teeth, and for the healing of wounds. It is used in the treatment of scurvy. Man is one of the few mammals unable to manufacture ascorbic acid in his liver. Used as a photographic developing agent in alkaline solution. 

Fig. 6. The far ultraviolet rotatory dispersion of native calf skin collagen - in 0.01 molar acetic solution. The ultraviolet rotatory dispersion of the same preparation of calf skin collagen heated at 50 °C for 30 min, coded to 25 °C and measured immediately, O-O-O. Concentrations were between 0.0076 and 0.076%. Data from Blout et al.18a)...

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