Collagen carbohydrate removal from

Kiihn ei al. (1959) attempted to remove the carbohydrate from acid-soluble collagen by two nondestructive methods (1) by repeatedly dissolving and reprecipitating the fibers by dialysis, and (2) by repeated extraction of the soluble collagen solution with chloroform whereby the protein separates out at the water/chloroform interface while free carbohydrate remains in the aqueous layer. They confirmed that all the glucosamine and half the hexose could be removed by either method, but the remaining 0.5 % of hexose could not be reduced further by repeated use of either procedure. 

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