Coiled coils stability

Scheme 2 shows a cross-sectional representation of a two-stranded a-helical coiled coil of 35 residues per polypeptide chain. The design of this idealized coiled coil incorporated the factors that maximize coiled-coil stability, for example, the hydrophobicity and packing effects in the hydrophobic core, intrachain electrostatic attractions, helical propensity contribution s[2324 from residues outside of the hydrophobic interface, and interchain electrostatic interactions.125 ... 

Effect of Polypeptide Chain Length on Coiled-Coil Stability... 

Second, what type of residues are best at the a and d sites This is also a difficult question to answer direcdy because, as will be addressed in more detail over the next few sections, the nature of residues at these sites influences coiled-coil stability, oligomer state, partner selection, and helix-helix orientation (Table I). However, in general terms, natural coiled-coil sequences tend to use the aliphatic hydrophobic residues (Ala, lie, Leu, Met, and Val) at these positions, rather than the aromatic hydrophobic side chains (Phe, Trp and Tyr) (Parry, 1982 Woolfson and Alber, 1995). The reason for this is probably a combination of bulk and steric constraints presented by the aromatic residues. However, a thorough understanding of the possible exclusion of aromatic side chains from coiled-coil... 

Burkhard, P., Ivaninskii, S., and Lustig, A. (2002). Improving coiled-coil stability by optimizing ionic interactions./. Mol. Biol. 318, 901-910. 

Lavigne, P., Sonnichsen, F. D., Kay, C. M., and Hodges, R. S. (1996). Interhelical salt bridges, coiled-coil stability, and specificity of dimerization. Science 271, 1136-1137. 

McClain, D. L., Gurnon, D. G., and Oakley, M. G. (2002). Importance of potential interhelical salt-bridges involving interior residues for coiled-coil stability and quaternary structure./. Mol Biol 324, 257-270. 

Kwok SC, Hodges RS. Effect of chain length on coiled-coil stability decreasing stabihty with increasing chain length. Biopolymers (Pept. Sci.) 2004 76 378-390. 

Yu YB. Coiled-coils stability, specificity, and drug delivery potential. Adv Drug Delivery Rev 2002 54 1113-1129. 

N 034 Helix-Coil Stability Constants for the Naturally Occurring Amino Acids In Water. I. Properties of Copolymers and Approximate Theories ... 

Scheraga, H. A. (1978). Use of random copolymers to determine the helix-coil stability constants of the naturally occurring amino acids. Pure Appl. Chem., 50 315. 

Sueki, M., Lee, S., Powers, S. R, Denton, J. B., Konishi, Y , Scheraga, H. A. (1984). Helix-coil stability constants for the naturally occurring amino acids in water. 22. Histidine parameters from rsmdom poly[(hydroxybutyl)glutamine-co-L-histidine],... 

The terminology host and guest originated in the field of organic conqilex-chemistry Scheraga was the first to introduce it into p tide chemistry he established the host-guest technique for the determination of helix-coil stability constants of all 20 naturally occurring a-amino adds 2-155) characteristic... 

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